Investigation on the Effect of Low Intensity Ultrasound Stimulation on Mouse Spermatogonial Stem Cell Proliferation and Colonization
Authors
Abstract:
Introduction: Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis. New procedure such as sound wave especially low intensity ultrasound (LIUS) can be effective on increasing the number of cells. In this study, the effect of LIUS stimulation on mouse SSCs proliferation and colonization was investigated. Methods: Isolated SSCs from neonatal mice were cultured in DMEM culture medium with 10% Fetal Bovine Serum (FBS). In the first phase of study, temperature controlled by LIUS of plate containing culture medium and in the next phase, SSCs stimulated by LIUS with 3 different Intensity doses (100, 200 and 300 mW/cm2) for 5 days and SSCs proliferation and colonization assessed at day 7. Results: The LIUS treatment of mouse SSCs increased the proliferation and colonization of SSCs in experimental groups compared to the control group. The average of proliferation in 100, 200, 300 mW/cm2 and control group were 1.96 ± 0.03, 2.26 ± 0.03, 1.73 ± 0.03 and 1.66 ± 0.03, respectively. The average number of colonies in 100, 200,300 mW/cm2 and control group were 45 ± 1.2, 53 ± 2.4, 28 ± 1.2 and 20 ± 0.8, respectively. The average diameters of colonies in 100, 200, 300 mW/cm2 and control group were 235.3 ± 6.8 &mum, 204.6 ± 12.3 &mum, 203.6 ± 5.6 and 214.3 ± 9.1 &mum, respectively. The results showed significant increase in proliferation rate and number of colonies in experimental groups compared to control group (P<0.05), whereas there were no significant difference between groups regarding to diameter of colonies. Conclusion: The results suggested that LIUS treatment can be an efficient and cost-effective method to improve the proliferation and colonization of SSCs during culture.
similar resources
P-103: Enhancement of Colonization on Mouse Spermatogonial Stem Cell by Low Intensity Ultrasound Stimulation
Background: Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis. New procedure such as sound wave especially low intensity ultrasound (LIUS) can be effective on increasing the number of cells. In this study we investigated the effect of LIUS stimulation on mouse SSCs. Materials and Methods: Isolated SSCs from neonate mice cultured in DMEM culture medium with 10% fetal bovine ...
full textIncrease of Proliferation and Colonization in Mouse Spermatogonial Stem Cell by Low Intensity Ultrasound
Background Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis. Sound wave especially low intensity ultrasound (LIUS) can be effective on increasing the number of cells. In this study we investigated the effect of LIUS stimulation on mouse SSCs proliferation and colonization. Methods: Isolated SSCs from neonate mice cultured in DMEM culture medium with 10% Fetal Bovine S...
full textincrease of proliferation and colonization in mouse spermatogonial stem cell by low intensity ultrasound
background spermatogonial stem cells (sscs) are the foundation of spermatogenesis. sound wave especially low intensity ultrasound (lius) can be effective on increasing the number of cells. in this study we investigated the effect of lius stimulation on mouse sscs proliferation and colonization. methods: isolated sscs from neonate mice cultured in dmem culture medium with 10% fetal bovine serum...
full textthe effect of low-intensity pulsed ultrasound stimulation on neonate mouse spermatogonial stem cells
objective: this study presents an efficient, cost-effective method to improve proliferation and colonization of spermatogonial stem cells (sscs) in vitro. methods: isolated sscs from neonate mice were cultured in dmem culture medium with 10% fetal bovine serum (fbs). in the first phase of the study, the temperature was controlled by low intensity pulsed ultrasound stimulation (lipus) of the pla...
full textan investigation of the impact of self monitoring on langauge teachers motivational practice and its effect on learners motivation
the central purpose of this study was to conduct a case study about the role of self monitoring in teacher’s use of motivational strategies. furthermore it focused on how these strategies affected students’ motivational behavior. although many studies have been done to investigate teachers’ motivational strategies use (cheng & d?rnyei, 2007; d?rnyei & csizer, 1998; green, 2001, guilloteaux & d?...
My Resources
Journal title
volume 10 issue None
pages 119- 124
publication date 2013-08
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023